AjaxControlToolkit CalendarExtender not working after publish

I am using the AjaxControlToolkit CalendarExtender in my web site and its working in the visual studio 2012 but after publish to IIS Calendar Extender control won't show !

asp.net code :

<asp:TextBox ID="txtFrom" runat="server" Width="200"></asp:TextBox>
<asp:Image ID="image1" runat="server" ImageUrl="~/Image/calendar.png" />
<ajaxToolkit:CalendarExtender ID="Calendar1" runat="server" TargetControlID="txtFrom" Format="dd.MM.yyyy" Enabled="true" PopupButtonID="image1" CssClass="cal_Theme1"></ajaxToolkit:CalendarExtender>

Web.config :

<configuration>
  <configSections>
    <!-- For more information on Entity Framework configuration, visit http://ift.tt/1eigFsq -->
    <section name="entityFramework" type="System.Data.Entity.Internal.ConfigFile.EntityFrameworkSection, EntityFramework, Version=6.0.0.0, Culture=neutral, PublicKeyToken=b77a5c561934e089" requirePermission="false" />
  </configSections>
  <connectionStrings>
    <add name="DefaultConnection" connectionString="Data Source=(LocalDb)\v11.0;AttachDbFilename=|DataDirectory|\aspnet-RechnungProgramm-20140827102620.mdf;Initial Catalog=aspnet-RechnungProgramm-20140827102620;Integrated Security=True" providerName="System.Data.SqlClient" />
    <add name="RechnungConnectionString" connectionString="Data Source=(LocalDB)\v11.0;AttachDbFilename=|DataDirectory|\Rechnung.mdf;Integrated Security=True" providerName="System.Data.SqlClient" />
  </connectionStrings>
  <system.web>
    <authentication mode="None" />
    <compilation debug="true" targetFramework="4.5">
      <assemblies>
        <add assembly="System.Design, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B03F5F7F11D50A3A" />
        <add assembly="System.Web.Extensions.Design, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31BF3856AD364E35" />
        <add assembly="System.Windows.Forms, Version=4.0.0.0, Culture=neutral, PublicKeyToken=B77A5C561934E089" />
      </assemblies>
    </compilation>
    <httpRuntime targetFramework="4.5" />
    <pages>
      <namespaces>
        <add namespace="System.Web.Optimization" />
        <add namespace="Microsoft.AspNet.Identity" />
      </namespaces>
      <controls>
        <add assembly="Microsoft.AspNet.Web.Optimization.WebForms" namespace="Microsoft.AspNet.Web.Optimization.WebForms" tagPrefix="webopt" />
      <add tagPrefix="ajaxToolkit" assembly="AjaxControlToolkit" namespace="AjaxControlToolkit" /></controls>
    </pages>
    <membership>
      <providers>
        <!--
          ASP.NET Membership is disabled in this template. Please visit the following link http://ift.tt/1xKWZUh to learn about the ASP.NET Membership support in this template
        -->
        <clear />
      </providers>
    </membership>
    <profile>
      <providers>
        <!--
          ASP.NET Membership Profile is disabled in this template. Please visit the following link http://ift.tt/1xKWZUh to learn about the ASP.NET Membership support in this template
        -->
        <clear />
      </providers>
    </profile>
    <roleManager>
      <!--
            ASP.NET Membership Role is disabled in this template. Please visit the following link http://ift.tt/1xKWZUh to learn about the ASP.NET Membership support in this template
        -->
      <providers>
        <clear />
      </providers>
    </roleManager>
    <!--
            If you are deploying to a cloud environment that has multiple web server instances,
            you should change session state mode from "InProc" to "Custom". In addition,
            change the connection string named "DefaultConnection" to connect to an instance
            of SQL Server (including SQL Azure and SQL  Compact) instead of to SQL Server Express.
      -->
    <sessionState mode="InProc" customProvider="DefaultSessionProvider">
      <providers>
        <add name="DefaultSessionProvider" type="System.Web.Providers.DefaultSessionStateProvider, System.Web.Providers, Version=1.0.0.0, Culture=neutral, PublicKeyToken=31bf3856ad364e35" connectionStringName="DefaultConnection" />
      </providers>
    </sessionState>
  </system.web>
  <system.webServer>
    <modules>
      <remove name="FormsAuthenticationModule" />
    </modules>
    <handlers>
      <add name="ScriptResource" preCondition="integratedMode" verb="GET,HEAD" path="ScriptResource.axd" type="System.Web.Handlers.ScriptResourceHandler, System.Web.Extensions, Version=4.0.0.0, Culture=neutral, PublicKeyToken=31bf3856ad364e35" />
    </handlers>
  </system.webServer>
  <runtime>
    <assemblyBinding xmlns="urn:schemas-microsoft-com:asm.v1">
      <dependentAssembly>
        <assemblyIdentity name="WebGrease" culture="neutral" publicKeyToken="31bf3856ad364e35" />
        <bindingRedirect oldVersion="0.0.0.0-1.5.2.14234" newVersion="1.5.2.14234" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="Microsoft.WindowsAzure.Storage" publicKeyToken="31bf3856ad364e35" culture="neutral" />
        <bindingRedirect oldVersion="0.0.0.0-2.1.0.4" newVersion="2.1.0.4" />
      </dependentAssembly>
      <dependentAssembly>
        <assemblyIdentity name="HtmlAgilityPack" publicKeyToken="bd319b19eaf3b43a" culture="neutral" />
        <bindingRedirect oldVersion="0.0.0.0-1.4.9.0" newVersion="1.4.9.0" />
      </dependentAssembly>
    </assemblyBinding>
  </runtime>
  <entityFramework>
    <defaultConnectionFactory type="System.Data.Entity.Infrastructure.LocalDbConnectionFactory, EntityFramework">
      <parameters>
        <parameter value="v11.0" />
      </parameters>
    </defaultConnectionFactory>
    <providers>
      <provider invariantName="System.Data.SqlClient" type="System.Data.Entity.SqlServer.SqlProviderServices, EntityFramework.SqlServer" />
    </providers>
  </entityFramework>
</configuration>

and master page ScriptManager :

 <form runat="server">
            <ajaxToolkit:ToolkitScriptManager runat="Server" EnableScriptGlobalization="true"
                    EnableScriptLocalization="true" ID="ScriptManager1" ScriptMode="Debug" CombineScripts="false" >
            <Scripts>
                <%--To learn more about bundling scripts in ScriptManager see http://ift.tt/1eMOMJk --%>
                <%--Framework Scripts--%>
                <asp:ScriptReference Name="jquery" />
                <asp:ScriptReference Name="bootstrap" />
                <asp:ScriptReference Name="respond" />
                <asp:ScriptReference Name="WebForms.js" Path="~/Scripts/WebForms/WebForms.js" />
                <asp:ScriptReference Name="WebUIValidation.js" Path="~/Scripts/WebForms/WebUIValidation.js" />
                <asp:ScriptReference Name="MenuStandards.js" Path="~/Scripts/WebForms/MenuStandards.js" />
                <asp:ScriptReference Name="GridView.js" Path="~/Scripts/WebForms/GridView.js" />
                <asp:ScriptReference Name="DetailsView.js" Path="~/Scripts/WebForms/DetailsView.js" />
                <asp:ScriptReference Name="TreeView.js" Path="~/Scripts/WebForms/TreeView.js" />
                <asp:ScriptReference Name="WebParts.js" Path="~/Scripts/WebForms/WebParts.js" />
                <asp:ScriptReference Name="Focus.js" Path="~/Scripts/WebForms/Focus.js" />
                <asp:ScriptReference Name="WebFormsBundle" />
                <%--Site Scripts--%>
            </Scripts>
            </ajaxToolkit:ToolkitScriptManager>

i am using AjaxControlToolkit.dll Version 4.5.7.1213

How can i fix this ?